Research: Bioprocess technology
Our lab integrates metabolomics and bioprocess engineering to optimize microbial fermentation processes, particularly focusing on recombinant protein production in E. coli using complex media.
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Spent Media Analysis and Fed-Batch Optimization
A cornerstone of our approach is the use of LC-MS/GC-MS–based spent media analysis to monitor nutrient consumption and byproduct formation during high cell density fed-batch fermentations. This data feeds into dynamic flux balance analysis (dFBA) using a metabolic model, allowing us to identify metabolic bottlenecks and nutrient limitations that arise during the bioprocess. Our initial study highlighted the critical role of amino acid pathways and energy metabolism in response to the increased demands of recombinant protein expression.
By leveraging these insights, we designed rational feeding strategies including targeted amino acid supplementation and process modification that led to significant increase in protein titer. This methodology accelerates bioprocess optimization and reduces the number of experimental trials compared to conventional design-of-experiments (DoE) approaches.
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Production of recombinant therapeutics and enzymes for biocatalysis
We have also established high cell density fermentation protocols for the efficient production of (i) therapeutic proteins such as PDGF, and (ii) enzymes relevant to industrial biocatalysis. These processes are optimized for protein yield and scalability. We have also established lab scale biocatalytic processes facilitating application of nitrilases and carbonyl reductases in green chemistry and pharmaceutical synthesis.
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Address
Department of Chemical Engineering,
IIT Bombay
Contact
Lab: +91 22 25767232
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